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nonspecific protein binding sites  (Proteintech)


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    Structured Review

    Proteintech nonspecific protein binding sites
    Nonspecific Protein Binding Sites, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonspecific+protein+binding+sites/c-Myc+Antibody/pm39688535-237-22-34
    Average 96 stars, based on 1299 article reviews
    nonspecific protein binding sites - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Purkinje cell microzones mediate distinct kinematics of a single movement
    Article Snippet: .. Then, sections were incubated for 90 min at RT in a solution of PBS/0.5%Triton-X100/10% normal horse serum to block nonspecific protein-binding sites, and incubated 48 h at 4 °C in a solution of PBS/0.4% Triton-X100/2% normal horse serum (NHS), with primary antibodies diluted as follows: KCTD12 , 1:500 (rabbit polyclonal, 15523-1-AP, ProteinTech), KCTD12 , 1:200 (mouse monoclonal, sc-271855, SantaCruz Biotech.), AldolaseC 1:500 (goat polyclonal, SC-12065, Santa Cruz Biotech.), Hsp25 1:1000 (rabbit polyclonal, SPA-801, Stressgen), GFP 1:1000 (chicken polyclonal, GFP-1020, Aves), RFP 1:1000 (guinea pig polyclonal, 390005, Synaptic Systems), Parvalbumin 1:7000 (rabbit polyclonal, PV-25, Swant), SST (chicken polyclonal, 366006, Synaptic Systems; and goat polyclonal, sc-7819, SantaCruz), Calretinin (mouse monoclonal, Swan 6B3-GEL), Tbr2 1:1000 (chicken polyclonal, AB15894, MiliP). .. After rinsing in PBS, sections were incubated for 2 h at RT in PBS/0.4% Triton-X100/2% NHS solution with secondary antibodies coupled with Alexa488, Cy3 or Cy5 (1:200, Jackson ImmunoResearch).

    Article Title: Discovery of N -Trifluoromethylated Noscapines as Novel and Potent Agents for the Treatment of Glioblastoma.
    Article Snippet: The search for new and effective chemotherapeutic agents for the treatment of glioblastoma (GBM) represents an unmet need in drug discovery.. Herein, a class of novel N-trifluoromethylated noscapines has been disclosed.. Among them, 9′-bromo-N-trifluoromethyl noscapine 15c displayed superior in vitro anti-GBM potency.

    Blocking Assay:

    Article Title: Purkinje cell microzones mediate distinct kinematics of a single movement
    Article Snippet: .. Then, sections were incubated for 90 min at RT in a solution of PBS/0.5%Triton-X100/10% normal horse serum to block nonspecific protein-binding sites, and incubated 48 h at 4 °C in a solution of PBS/0.4% Triton-X100/2% normal horse serum (NHS), with primary antibodies diluted as follows: KCTD12 , 1:500 (rabbit polyclonal, 15523-1-AP, ProteinTech), KCTD12 , 1:200 (mouse monoclonal, sc-271855, SantaCruz Biotech.), AldolaseC 1:500 (goat polyclonal, SC-12065, Santa Cruz Biotech.), Hsp25 1:1000 (rabbit polyclonal, SPA-801, Stressgen), GFP 1:1000 (chicken polyclonal, GFP-1020, Aves), RFP 1:1000 (guinea pig polyclonal, 390005, Synaptic Systems), Parvalbumin 1:7000 (rabbit polyclonal, PV-25, Swant), SST (chicken polyclonal, 366006, Synaptic Systems; and goat polyclonal, sc-7819, SantaCruz), Calretinin (mouse monoclonal, Swan 6B3-GEL), Tbr2 1:1000 (chicken polyclonal, AB15894, MiliP). .. After rinsing in PBS, sections were incubated for 2 h at RT in PBS/0.4% Triton-X100/2% NHS solution with secondary antibodies coupled with Alexa488, Cy3 or Cy5 (1:200, Jackson ImmunoResearch).

    Article Title: Discovery of N -Trifluoromethylated Noscapines as Novel and Potent Agents for the Treatment of Glioblastoma.
    Article Snippet: The search for new and effective chemotherapeutic agents for the treatment of glioblastoma (GBM) represents an unmet need in drug discovery.. Herein, a class of novel N-trifluoromethylated noscapines has been disclosed.. Among them, 9′-bromo-N-trifluoromethyl noscapine 15c displayed superior in vitro anti-GBM potency.

    Protein Binding:

    Article Title: Purkinje cell microzones mediate distinct kinematics of a single movement
    Article Snippet: .. Then, sections were incubated for 90 min at RT in a solution of PBS/0.5%Triton-X100/10% normal horse serum to block nonspecific protein-binding sites, and incubated 48 h at 4 °C in a solution of PBS/0.4% Triton-X100/2% normal horse serum (NHS), with primary antibodies diluted as follows: KCTD12 , 1:500 (rabbit polyclonal, 15523-1-AP, ProteinTech), KCTD12 , 1:200 (mouse monoclonal, sc-271855, SantaCruz Biotech.), AldolaseC 1:500 (goat polyclonal, SC-12065, Santa Cruz Biotech.), Hsp25 1:1000 (rabbit polyclonal, SPA-801, Stressgen), GFP 1:1000 (chicken polyclonal, GFP-1020, Aves), RFP 1:1000 (guinea pig polyclonal, 390005, Synaptic Systems), Parvalbumin 1:7000 (rabbit polyclonal, PV-25, Swant), SST (chicken polyclonal, 366006, Synaptic Systems; and goat polyclonal, sc-7819, SantaCruz), Calretinin (mouse monoclonal, Swan 6B3-GEL), Tbr2 1:1000 (chicken polyclonal, AB15894, MiliP). .. After rinsing in PBS, sections were incubated for 2 h at RT in PBS/0.4% Triton-X100/2% NHS solution with secondary antibodies coupled with Alexa488, Cy3 or Cy5 (1:200, Jackson ImmunoResearch).

    Article Title: Discovery of N -Trifluoromethylated Noscapines as Novel and Potent Agents for the Treatment of Glioblastoma.
    Article Snippet: The search for new and effective chemotherapeutic agents for the treatment of glioblastoma (GBM) represents an unmet need in drug discovery.. Herein, a class of novel N-trifluoromethylated noscapines has been disclosed.. Among them, 9′-bromo-N-trifluoromethyl noscapine 15c displayed superior in vitro anti-GBM potency.

    Staining:

    Article Title: Discovery of N -Trifluoromethylated Noscapines as Novel and Potent Agents for the Treatment of Glioblastoma.
    Article Snippet: The search for new and effective chemotherapeutic agents for the treatment of glioblastoma (GBM) represents an unmet need in drug discovery.. Herein, a class of novel N-trifluoromethylated noscapines has been disclosed.. Among them, 9′-bromo-N-trifluoromethyl noscapine 15c displayed superior in vitro anti-GBM potency.



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